Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1397
10
1
gregorian
2019
1
1
10
1
online
1
fulltext
en
Characterization of Functional Effects of Two New Active Fractions Isolated From Scorpion Venom on Neuronal Ca2+ Spikes: A Possible Action on Ca2+-Dependent Dependent K+ Channels
Behavioral Neuroscience
Behavioral Neuroscience
Original
Original
<div style="text-align: justify;"><strong>Introduction:</strong> It is a long time that natural toxin research is conducted to unlock the medical potential of toxins. Although venoms-toxins cause pathophysiological conditions, they may be effective to treat several diseases. Since toxins including scorpion toxins target voltage-gated ion channels, they may have profound effects on excitable cells. Therefore, elucidating the cellular and electrophysiological impacts of toxins, particularly scorpion toxins would be helpful in future drug development opportunities.<br>
<strong>Methods:</strong> Intracellular recording was made from F1 cells of Helix aspersa in the presence of calcium Ringer solution in which Na+ and K+ channels were blocked. Then, the modulation of channel function in the presence of extracellular application of F4 and F6 toxins and kaliotoxin (KTX; 50 nM and 1 μM) was examined by assessing the electrophysiological characteristics of calcium spikes.<br>
<strong>Results:</strong> The two active toxin fractions, similar to KTX, a known Ca2+-activated K+ channel blocker, reduced the amplitude of AHP, enhanced the firing frequency of calcium spikes and broadened the duration of Ca2+ spikes. Therefore, it might be inferred that these two new fractions induce neuronal hyperexcitability possibly, in part, by blocking calcium-activated potassium channel current. However, this supposition requires further investigation using voltage clamping technique.<br>
<strong>Conclusion:</strong> These toxin fractions may act as blocker of calcium-activated potassium channels.</div>
Scorpion Venom, Intracellular recording, Calcium spike, Buthotus schach
49
58
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1247-1&slc_lang=en&sid=1
Hanieh
Tamadon
tmdn.hanieh@gmail.com
13700319475328460022372
13700319475328460022372
No
Department of Physiology, Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Zahra
Ghasemi
ghasemi.z1985@gmail.com
13700319475328460022373
13700319475328460022373
No
Department of Physiology, School of Medicine, Tarbiat Modares University, Tehran, Iran.
Fatemeh
Ghasemi
ghasemikh2012@gmail.com
13700319475328460022374
13700319475328460022374
No
Department of Physiology, Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Narges
Hosseinmardi
nargeshosseinmardi@gmail.com
13700319475328460022375
13700319475328460022375
No
Department of Physiology, Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Hossein
Vatanpour
vatanpour.hossein@gmail.com
13700319475328460022376
13700319475328460022376
No
Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mahyar
Janahmadi
Janahmadi@sbmu.ac.ir
13700319475328460022377
13700319475328460022377
Yes
Department of Physiology, Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.