Basic and Clinical Neuroscience Journal
مجله علوم اعصاب پایه و بالینی
BCN
Medical Sciences
http://bcn.iums.ac.ir
137
journal137
2008-126X
2228-7442
10.32598/bcn
en
jalali
1398
6
1
gregorian
2019
9
1
10
5
online
1
fulltext
en
A 6-step Approach to Gain Higher Quality Results From Organotypic Hippocampal Brain Slices in a Traumatic Brain Injury Model
Cellular and molecular Neuroscience
Cellular and molecular Neuroscience
Original
Original
<div style="text-align: justify;"><strong>Introduction: </strong>Organotypic Hippocampal Brain Slices (OHBS) provide an advantageous alternative to in vivo models to scrutinize Traumatic Brain Injury (TBI). We followed a well-established TBI protocol, but noticed that several factors may influence the results in such a set-up. Here, we describe a structured approach to generate more comparable results and discuss why specific eligibility criteria should be applied.<br>
<strong>Methods: </strong>We defined necessary checkpoints and developed inclusion and exclusion criteria that take the observed variation in such a model into consideration. Objective measures include the identification and exclusion of pre-damaged slices and outliers. Six steps were outlined in this study.<br>
<strong>Results: </strong>A six-step approach to enhance comparability is proposed and summarized in a flowchart. We applied the suggested measures to data derived from our TBI-experiments examining the impact of three different interventions in 1459 OHBS. Our exemplary results show that through equal requirements set for all slices more precise findings are ensured.<br>
<strong>Conclusion:</strong> Results in a TBI experiment on OHBS should be analyzed critically as inhomogeneities may occur. In order to ensure more precise findings, a structured approach of comparing the results should be followed. Further research is recommended to confirm and further develop this framework.</div>
Organotypic hippocampal brain slices, In vitro model, Traumatic Brain Injury (TBI), Propidium Iodide (PI), Eligibility, Framework
485
498
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1213-1&slc_lang=en&sid=1
Linda
Grüßer
linda.gruesser@googlemail.com
13700319475328460030846
13700319475328460030846
Yes
Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany.
Rosmarie
Blaumeiser-Debarry
Rblaumeiser-debarry@ukaachen.de
13700319475328460030847
13700319475328460030847
No
Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany.
Rolf
Rossaint
rrossaint@ukaachen.de
13700319475328460030848
13700319475328460030848
No
Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany.
Matthias
Krings
matthias.krings@rwth-aachen.de
13700319475328460030849
13700319475328460030849
No
Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany.
Benedikt
Kremer
benedikt.kremer@rwth-aachen.de
13700319475328460030850
13700319475328460030850
No
Department of Neurosurgery, RWTH Aachen University Hospital, Pauwelsstr. 30, 52074 Aachen, Germany
Anke
Höllig
ahoellig@ukaachen.de
13700319475328460030851
13700319475328460030851
No
Department of Neurosurgery, RWTH Aachen University Hospital, Aachen, Germany.
Mark
Coburn
mcoburn@ukaachen.de
13700319475328460030852
13700319475328460030852
No
Department of Anesthesiology, RWTH Aachen University Hospital, Aachen, Germany.