TY - JOUR T1 - Diagnostic Challenges and Prospects Associated With Zoonotic Tuberculosis of Central Nervous System TT - JF - BCN JO - BCN VL - 11 IS - 5 UR - http://bcn.iums.ac.ir/article-1-874-en.html Y1 - 2020 SP - 619 EP - 630 KW - Duplex PCR KW - Mycobacterium Bovis KW - Tuberculous meningitis KW - Zoonoses N2 - Introduction: The diagnosis of Tuberculous Meningitis (TBM) has remained a challenge due to its insidious onset and the failure of conventional diagnostic tests. The present study aimed to identify the mycobacterial pathogen in the CSF of patients with TBM and a poor prognosis. Methods: We retrospectively recruited 224 TBM and 34 non-TBM patients admitted to the Central India Institute of Medical Sciences, Nagpur, India, in 2014. The CSF samples of these patients were subjected to a duplex PCR assay for the species-specific identification of the causative pathogen. Results: M. bovis and infection with M.tuberculosis were detected in 7% (18) and 32.9% (85) of the patients, respectively. Moreover, 14% (36) of the study samples were culture positive; however, the mycobacterial pathogens could not be differentiated to the species level. Conclusion: The present study findings emphasized the potentially vital importance of M. bovis identification for appropriate patient management. The obtained data also demonstrated the persistent significance of M. bovis, as a zoonotic pathogen. M3 10.32598/bcn.9.10.11.5.803.3 ER -