AU - Azizbeigi, Ronak AU - Farzinpour, Zahra AU - Haghparast, Abbas TI - Role of Orexin-1 Receptor Within the Ventral Tegmental Area in Mediating Stress- and Morphine Priming-induced Reinstatement of Conditioned Place Preference in Rats PT - JOURNAL ARTICLE TA - BCN JN - BCN VO - 10 VI - 4 IP - 4 4099 - http://bcn.iums.ac.ir/article-1-1107-en.html 4100 - http://bcn.iums.ac.ir/article-1-1107-en.pdf SO - BCN 4 ABĀ  - Introduction: Orexin-containing neurons exist in the lateral hypothalamic region, sending their projections toward mesolimbic regions such as the Ventral Tegmental Area (VTA). Methods: In the current study, a Reinstatement model is used to examine the effects of intra-VTA administration of SB334867 as an Orexin-1 Receptor (OX1R) antagonist on drug priming- and Forced Swim Stress (FSS)-induced reinstatement of morphine. Eighty-eight male adult albino Wistar rats, weighing 200-280 g, were bilaterally implanted by cannulas into the VTA. We induced the Conditioned Place Preference (CPP) by Subcutaneous (SC) injection of morphine (5 mg/kg) daily in three days. Then, the CPP score was calculated. After a 24-h “off” period following achievement of extinction criterion, the rats were tested for drug priming-induced reinstatement by a priming dose of morphine (1 mg/kg, SC) and for FSS-induced reinstatement 10 min after FSS. In the next experiments, the animals received different doses of intra-VTA administration of SB334867 (0.3, 3, and 1 nM/0.3 µL 12% DMSO per side) and bilaterally were subsequently tested for FSS- and morphine priming-induced reinstatement. Results: Our findings indicated that the FSS could induce the reinstatement of seeking behaviors. Furthermore, intra-VTA administration of OX1R antagonists suppressed FSS- and drug priming-induced reinstatement dose-dependently. Conclusion: It is concluded that FSS and drug priming-induced reinstatement might be mediated, at least in part, by stimulation of orexin receptors in the VTA. CP - IRAN IN - Department of physiology, Faculty of Veterinary Medicine LG - eng PB - BCN PG - 373 PT - Original YR - 2019