@ARTICLE{Parvishan, author = {Parvishan, Asghar and Taslimil, Zahra and Ebrahimzadeh, Mohammad and Haghparast, Abbas and }, title = {Capsazepine, a Transient Receptor Potential Vanilloid Type 1 (TRPV1) Antagonist, Attenuates Antinociceptive Effect of CB1 Receptor agonist, WIN55,212-2, in the Rat Nucleus Cuneiformis}, volume = {2}, number = {4}, abstract ={Introduction: Nucleus cuneiformis (NCF), as part of descending pain inhibitory system, cooperates with periaqueductal gray (PAG) and rostral ventromedial medulla (RVM) in supraspinal modulation of pain. Cannabinoids have analgesic effects in the PAG, RVM and NCF. The transient receptor potential vanilloid type 1(TRPV1) can be activated by anandamide and WIN55,212-2 as a cannabinoid receptor agonist. The aim of the current study is to investigate the possible interplay between the cannabinoid and vanilloid systems for modulation of pain at the NCF. Methods: In this study, a cannabinoid receptor agonist, WIN55,212-2 ( 15 μg/0.3 μl DMSO), and selective TRPV1 receptor antagonist, capsazepine (10, 25, 50 and100 nmol/0.3 μl DMSO), were microinjected bilaterally into the NCF, and tail-flick and formalin tests were used to assess the animal’s pain-related behaviors at 5-min intervals for a 60-min period. Results: Our findings demonstrated that analgesic effect of WIN55,212-2 were dose-dependently attenuated by capsazepine in both tests. In the tail-flick test, capsazepine at both doses of 50 (P }, URL = {http://bcn.iums.ac.ir/article-1-175-en.html}, eprint = {http://bcn.iums.ac.ir/article-1-175-en.pdf}, journal = {Basic and Clinical Neuroscience Journal}, doi = {}, year = {2011} }