TY - JOUR T1 - Evaluation of the Functional Recovery in Sciatic Nerve Injury following the Co-transplantation of Schwann and Bone Marrow Stromal Stem Cells in Rat TT - JF - BCN JO - BCN VL - 4 IS - 4 UR - http://bcn.iums.ac.ir/article-1-432-en.html Y1 - 2013 SP - 291 EP - 298 KW - Bone Marrow Stromal Cells KW - Schwann Cells KW - Transplantation KW - Peripheral Nerve Regeneration N2 - Introduction: Transplantation of bone marrow stromal cells (BMSCs) or Schwann cells (SCs) can increase axonal regeneration in peripheral nerve injuries. Based on our previous investigations, the goal of the present work was to examine the individual and synergistic effects of the two different cell types in sciatic nerve injury . We pursued to evaluate the effects of BMSCs and SCs co-transplantation on the functional recovery after sciatic nerve injury in rat. Methods: In this experimental research, adult male Wistar rats (n=32, 250-300g) were used, BMSCs and SCs were cultured, and the SCs were confirmed with anti S100 antibody. Rats were randomly divided into 4 groups (n=8 in each group): 1- control group: silicon tube filled with fibrin gel without cells 2- BMSCs group: silicon tube filled with fibrin gel seeded with BMSCs 3- SCs group: silicon tube filled with fibrin gel seeded with SCs and 4- co-transplantation group: silicone tube filled with fibrin gel seeded with BMSCs and SCs. The left sciatic nerve was exposed, a 10 mm segment removed, and a silicone tube interposed into this nerve gap. BMSCs and SCs were transplanted separately or in combination into the gap. BMSCs were labeled with anti-BrdU and SCs were labeled with DiI. After 12 weeks electromyographic and functional assessments were performed and analyzed by one-way analysis of variance (ANOVA). Results: Electromyographic and functional assessments showed a significant difference between the experimental groups and controls. Electromyography measures were significantly more favourable in SCs transplantation group as compared to BMSCs transplantation and co-transplantation groups (p M3 ER -