Accepted Articles                   Back to the articles list | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khodayari S, Ghaderi Pakdel F, Shahabi P, Naderi S. Acute Tramadol-induced cellular tolerance and dependence of ventral tegmental area dopaminergic neurons: An in vivo electrophysiological study. BCN. 2017;
URL: http://bcn.iums.ac.ir/article-1-981-en.html
1- Department of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
2- Associate Professor Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran.
3- Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4- Danesh Pey Hadi Co, Health and Technology Development Center, Urmia University of Medical Sciences, Urmia, Iran
Abstract:  

Introduction: Ventral tegmental area (VTA) is a core region of brainstem that contributes in different vital bio-responses such as pain and addiction. The dopaminergic (DA) cellular content of the VTA revealed have major roles in different functions. The cellular effect of the tramadol on the putative VTA-DA neurons evaluated in this study.
Methods: Wistar rats classified into three control, sham, and tramadol-treated groups. Animals anesthetized and VTA-DA neuronal activity acquisitioned under controlled stereotaxic surgery. The firing rate of the neurons extracted according principle component analysis by Igor pro software and analysed statistically with p<0.05. Tramadol (20 mg/kg) infused intraperitoneally.
Results: Overall, 121 putative VTA-DA neurons isolated in all groups. In tramadol-treated rats, inhibition of the neuronal firing as tolerance and constitutive excitation period as dependence or withdrawal observed. The inhibition lasted up to 50.34±10.17 minutes and 31% of neurons abolished firing and silenced for 24±3 min but the remaining lowered their firing up to 43% to 67% of their baseline firing. All neurons showed the excitation period that lasted about 56.12±15.30 min and the firing of neurons raised up their firing from 176% up to 244% of their baseline or pre-injection period.
Conclusion: The tolerance and dependence effects of tramadol relate to the change of neuronal firing rate at the putative VTA-DA neurons. The acute injection of tramadol can initiate neuroadaptation on the opioid and non-opioid neurotransmission to mediate these effects.

Type of Study: Original | Subject: Behavioral Neuroscience
Received: 2017/07/13 | Accepted: 2018/04/30 | Published: 2018/05/13

Add your comments about this article : Your username or Email:
Write the security code in the box

Send email to the article author


© 2015 All Rights Reserved | Basic and Clinical Neuroscience

Designed & Developed by : Yektaweb