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1- Department of Pharmacology and Therapeutics, University of Ibadan

Introduction: Isoniazid-induced seizure, often described as status epilepticus (SE), is a life-threatening condition, which is characterized by prolonged convulsions and responds poorly to currently available anti-convulsant drugs. This study was designed to evaluate the effects of Jobelyn®, an African-based dietary supplement on seizures, altered oxidative stress and glutamate decarboxylase activity induced by isoniazid in mice.
Methods: Mice (n = 6) received oral administration of JB (10, 25 and 50 mg/kg), pyridoxine (300 mg/kg), diazepam (5 mg/kg) or distilled water (10 mL/kg) 30 min before induction of SE with intraperitoneal injection of isoniazid (300 mg/kg). Thereafter, the animals were observed for the onset of convulsions for a period of 2 h. The effect of JB (10-50 mg/kg) on glutamate decarboxylase (GAD) activity and biomarkers of oxidative stress [glutathione (GSH) and malondialdehyde (MDA)] were also evaluated in the brain homogenates of another set of isoniazid-treated mice.
Results: JB (50 mg/kg, p.o) delayed the onset of isoniazid-induced convulsions but could not prevent the occurrence of seizure episodes. Moreover, JB did not show any protection against death nor delay the latency to death caused by isoniazid. The brain levels of MDA and GSH in isoniazid-treated mice were positively modulated by JB (50 mg/kg, p.o). The activity of GAD, an enzyme responsible for GABA synthesis was increased by JB, which suggest enhanced GABAergic neurotransmission.  
Discussion: The results of this study suggest that JB delayed the onset of isoniazid-induced convulsions, enhanced GABAergic neurotransmission and also demonstrated anti-oxidative effect in mice.
Type of Study: Original | Subject: Behavioral Neuroscience
Received: 2017/07/13 | Accepted: 2018/03/3 | Published: 2018/05/26

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