دوره 7، شماره 3 - ( Summer 2016 -- 1395 )                   جلد 7 شماره 3 صفحات 231-240 | برگشت به فهرست نسخه ها




DOI: 10.15412/J.BCN.03070308
PMID: 27563416
PMCID: PMC4981835

Cited 0 times in PubMed Central

XML English Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Gazerani S, Zaringhalam J, Manaheji H, Golabi S. The Role of C Fibers in Spinal Microglia Induction and Possible Relation with TRPV3 Expression During Chronic Inflammatory Arthritis in Rats. BCN. 2016; 7 (3) :231-240
URL: http://bcn.iums.ac.ir/article-1-650-fa.html
The Role of C Fibers in Spinal Microglia Induction and Possible Relation with TRPV3 Expression During Chronic Inflammatory Arthritis in Rats. مجله علوم اعصاب پایه و بالینی. 1395; 7 (3) :231-240

URL: http://bcn.iums.ac.ir/article-1-650-fa.html


چکیده:  

Introduction: Stimulation of peptidergic fibers activates microglia in the dorsal horn. Microglia activation causes fractalkine (FKN) release, a neuron-glia signal, which enhances pain. The transient vanilloid receptor 1 (TRPV1) mediates the release of neuropeptides, which can subsequently activate glia. TRPV1 and TRPV2 are generally expressed on C and Aδ fibers, respectively. Expression of both proteins is upregulated during inflammation, but expression of TRPV3 after induction of inflammation is unclear.
Methods: Adult male Wistar rats were used in all experiments. Arthritis was induced in them by single subcutaneous injection of complete Freund’s adjuvant (CFA) in their right hindpaws. Resiniferatoxin (RTX) was used to eliminate peptidergic fibers. We examined the relation between FKN and TRPV3 expression by administration of anti-FKN antibody.
Results: Our study findings indicated that 1) spinal TRPV3 was mostly expressed on nonpeptidergic fibers, 2) expression of spinal TRPV3 increased following inflammation, 3) elimination of peptidergic fibers decreased spinal TRPV3 expression, 4) alteration of hyperalgesia was compatible with TRPV3 changes in RTX-treated rat, and 5) anti-FKN antibody reduced spinal TRPV3 expression.
Conclusion: It seems that the hyperalgesia variation during different phases of CFA-induced arthritis correlates with spinal TRPV3 expression variation on peptidergic fibers. Moreover, spinal microglial activation during CFA inflammation is involved in TRPV3 expression changes via FKN signaling.

نوع مطالعه: Original | موضوع مقاله: Cellular and molecular Neuroscience
دریافت: ۱۳۹۳/۱۲/۱۱ | پذیرش: ۱۳۹۴/۶/۵ | انتشار: ۱۳۹۵/۴/۱۱

کلیه حقوق این وب سایت متعلق به Basic and Clinical Neuroscience می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2015 All Rights Reserved | Basic and Clinical Neuroscience

Designed & Developed by : Yektaweb