Volume 8, Issue 6 (November & December 2017)                   BCN 2017, 8(6): 453-466 | Back to browse issues page


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Vishwakarma S K, Bardia A, Fathima N, Chandrakala L, Rahamathulla S, Raju N, et al . Protective Role of Hypothermia Against Heat Stress in Differentiated and Undifferentiated Human Neural Precursor Cells: A Differential Approach for the Treatment of Traumatic Brain Injury. BCN. 2017; 8 (6) :453-466
URL: http://bcn.iums.ac.ir/article-1-647-en.html

1- Central Laboratory for Stem Cell Research and Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Hyderabad, India.
2- Department of Genetics, Faculty of Science, Osmania University, Hyderabad, India.
3- Centre for Cellular and Molecular Biology, Hyderabad, India.
4- PhD Central Laboratory for Stem Cell Research and Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Hyderabad, India.
Abstract:  
Introduction: The present study aimed to explore protective mechanisms of hypothermia against mild cold and heat stress on highly proliferative homogeneous human Neural Precursor Cells (NPCs) derived from Subventricular Zone (SVZ) of human fetal brain. 
Methods: CD133+ve enriched undifferentiated and differentiated human NPCs were exposed to heat stress at 42°C. Then, Western-blot quantification was performed using Hsp-70 (70 kilodalton heat shock proteins) recombinant protein. Finally, changes in pluripotency and Hsp-70 expression were measured using immunofluorescence staining and RT-qPCR (Quantitative reverse transcription PCR) analysis, respectively. 
Results: Heat stress resulted in abnormal neurospheres development. The apoptosis rate was enhanced during long-term in vitro culture of neurospheres. Neurogenic differentiation reduced and showed aberrent phenotypes during heat stress. After hypothermia treatment significant improvement in neurospheres and neuronal cell morphology was observed. 
Conclusion: Mild-hypothermia treatment induces attenuated heat shock response against heat stress resulting in induced HSP-70 expression that significantly improves structure and function of both undifferentiated human NPCs and differentiated neurons.
Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2016/06/27 | Accepted: 2017/02/7 | Published: 2017/11/1

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