دوره 6، شماره 3 - ( Summer 2015 -- 1394 )                   جلد 6 شماره 3 صفحات 171-178 | برگشت به فهرست نسخه ها



PMID: 26904174
PMCID: PMC4656990

Cited 0 times in PubMed Central

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Eftekhar-Vaghefi S, Esmaeili Mahani S, Elyasi L, Abbasnejad M. Involvement of Mu Opioid Receptor Signaling in The Protective Effect of Opioid against 6-Hydroxydopamine-Induced SH-SY5Y Human Neuroblastoma Cells Apoptosis. BCN. 2015; 6 (3) :171-178
URL: http://bcn.iums.ac.ir/article-1-548-fa.html
Involvement of Mu Opioid Receptor Signaling in The Protective Effect of Opioid against 6-Hydroxydopamine-Induced SH-SY5Y Human Neuroblastoma Cells Apoptosis. مجله علوم اعصاب پایه و بالینی. 1394; 6 (3) :171-178

URL: http://bcn.iums.ac.ir/article-1-548-fa.html


چکیده:  
Introduction: The neuroprotective role of opioid morphine against 6-hydroxydopamineinduced cell death has been demonstrated. However, the exact mechanism(s) underlying such neuroprotection, especially the role of subtype receptors, has not yet been fully clarified. 
Methods: Here, we investigated the effects of different opioid agonists on 6-OHDA-induced neurotoxicity in human neuroblastoma SH-SY5Y cell line as an in vitro model of Parkinson’s disease. Cell damage was induced by 150 μM 6-OHDA and the cells viability was examined by MTT assay. Intracellular calcium, reactive oxygen species and mitochondrial membrane potential were assessed by fluorescence spectrophotometry method. Immunoblot technique was used to evaluate cytochrome-c and activated caspase-3 as biochemical markers of apoptosis induction. 
Results: The data showed that 6-OHDA caused significant cell damage, loss of mitochondrial membrane potential and increase in intracellular reactive oxygen species and calcium levels as well as activated caspase-3 and cytochrome-c release. Incubation of SH-SY5Y cells with μ-opioid agonists, morphine and DAMGO, but not with δ-opioid agonist, DADLE, elicited protective effect and reduced biochemical markers of cell damage and death. 
Discussion: The results suggest that μ-opioid receptors signaling participate in the opioid neuroprotective effects against 6-OHDA-induced neurotoxicity.
نوع مطالعه: Original | موضوع مقاله: Behavioral Neuroscience
دریافت: ۱۳۹۳/۸/۲۰ | پذیرش: ۱۳۹۴/۲/۳۱ | انتشار: ۱۳۹۴/۴/۱۰

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