دوره 1، شماره 2 - ( Winter 2010 -- 1388 )                   جلد 1 شماره 2 صفحات 37-42 | برگشت به فهرست نسخه ها



PMCID: 17391650

Cited 0 times in PubMed Central

XML English Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Marzban M, Bakhtiary M, Mehdizadeh M, Taghi Joghataei M, Khoei S, Pirhajati Mahabadi V et al . Mobilization of stem cell with granulocyte-colony stimulating factor promotes recovery after traumatic brain injury in rat. BCN. 2010; 1 (2) :37-42
URL: http://bcn.iums.ac.ir/article-1-30-fa.html
Mobilization of stem cell with granulocyte-colony stimulating factor promotes recovery after traumatic brain injury in rat. مجله علوم اعصاب پایه و بالینی. 1388; 1 (2) :37-42

URL: http://bcn.iums.ac.ir/article-1-30-fa.html


چکیده:  

Introduction: This study was designed to investigate the effects of granulocyte colony-stimulating factor (G-CSF) administration in rats for 6 weeks after traumatic brain injury (TBI).

Methods: Adult male Wistar rats (n = 30) were injured with controlled cortical impact device and divided into four groups. The treatment groups (n = 10 each) were injected subcutaneously with recombinant human G-CSF. Vehicle group (n=10) received phosphate buffered saline (PBS) and only Brdu intraperitoneally. Bromodeoxyuridine (BrdU) was used for mitotic labeling. Experimental rats were injected intraperitoneally with BrdU. Rats were killed at 6th week after traumatic brain injury. Neurological functional evaluation of animals was performed before and after injury using neurological severity scores (NSS). Animals were sacrificed 42 days after TBI and brain sections were stained using Brdu immunohistochemistry.

Results: Statistically significant improvement in functional outcome was observed in treatment groups when compared with control (p<0.01). This benefit was visible 7 days after TBI and persisted until 42 days (end of trial). Histological analysis showed that Brdu cell positive was more in the lesion boundary zone at treatment animal group than all injected animals.

Discussion: We believe that G-CSF therapeutic protocol reported here represents an attractive strategy for the development of a clinically significant noninvasive traumatic brain injury therapy.

نوع مطالعه: Original |
دریافت: ۱۳۸۸/۵/۵ | پذیرش: ۱۳۹۵/۶/۹ | انتشار: ۱۳۹۵/۶/۹

کلیه حقوق این وب سایت متعلق به Basic and Clinical Neuroscience می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2015 All Rights Reserved | Basic and Clinical Neuroscience

Designed & Developed by : Yektaweb